Journal: JCI Insight

Article Title: Role of the mitochondrial protein cyclophilin D in skin wound healing and collagen secretion

doi: 10.1172/jci.insight.169213

Figure Lengend Snippet: ( A ) Illustration of treatments and experiments conducted. ( B and C ) H&E-stained sections and quantification (mean ± SEM) of explant skin wounds at different days after wounding as indicated after transdermal treatment with the specific CyD inhibitor NIM811. Arrows indicate wound size by marking epidermal edges. n =3. Magnification, ×4. ( D and E ) Scratch wound assay of keratinocytes and human primary fibroblasts treated with NIM811. ( F ) qPCR analysis (mean ± SEM) of PPIF (CyD) and COL1A1 (collagen 1) expression in explants after transdermal treatment with NIM811 for 7 days. ( G ) Collagen level (mean ± SEM) by hydroxyproline assay after different days of NIM811 treatment. n = 3 for all experiments. *** P < 0.001; * P < 0.05.

Article Snippet: For assessing the collagen deposition in healthy and NIM811-treated human skin samples, we used hydroxyproline assay kit (Chondrex).

Techniques: Staining, Scratch Wound Assay Assay, Expressing, Hydroxyproline Assay

Journal: Advanced Science

Article Title: Macrophage–Derived Ferritin Exacerbates Silica‐Induced Pulmonary Fibrosis via PIK3R2‐Mediated Fibroblast Differentiation

doi: 10.1002/advs.202519191

Figure Lengend Snippet: Exogenous ferritin aggravates silica‐induced pulmonary fibrosis in mice. A) Schematic of in vivo study design. Mice were given an intratracheal infusion of silica, followed by daily intravenous ferritin for 7 days. B) Representative H&E staining of lung tissue from mice co‐exposed to silica and ferritin (magnification, 2×, 20×). C) Representative Masson's trichrome staining of lung tissue from co‐exposed mice (magnification, 2×, 20×). D) Quantification of collagen volume fraction based on Masson's trichrome staining (n = 9 per group). E) Hydroxyproline content in lung tissues from silica and ferritin co‐exposed mice (n = 4 or 5 per group). F) Protein expression of Collagen I, Collagen III, and fibronectin detected by Western blot. G–I) Quantitative analysis of Collagen I, Collagen III, and fibronectin protein levels (n = 3 per group). J, K) Heatmaps of inflammation‐ and fibrosis‐related genes in lung tissue from silica and ferritin co‐exposed mice. Data are presented as mean ± SD, * p < 0.05, ** p < 0.01, *** p < 0.001. Statistical analysis was performed using one‐way ANOVA followed by Dunnett's test (G, I) and LSD (D, E, H).

Article Snippet: Approximately 10 mg of lung tissue was weighed and hydrolyzed in 6 mol/L hydrochloric acid at 95°C for 6 h. Hydroxyproline levels were subsequently quantified using a commercial hydroxyproline assay kit (Elabscience, E‐BC‐K062‐M) following the manufacturer's protocol.

Techniques: In Vivo, Staining, Expressing, Western Blot

Journal: Advanced Science

Article Title: Macrophage–Derived Ferritin Exacerbates Silica‐Induced Pulmonary Fibrosis via PIK3R2‐Mediated Fibroblast Differentiation

doi: 10.1002/advs.202519191

Figure Lengend Snippet: Reduced ferritin expression alleviates silica‐induced pulmonary fibrosis in mice. A) Schematic of in vivo study design. Mice were given an intratracheal infusion of AAV6 carrying Ftl1 ‐specific shRNA. After two weeks of infection, silica was administered intratracheally, and lung tissues were collected after four weeks of silica exposure. B) Lung weight coefficients in silica‐induced pulmonary fibrosis mice with or without ferritin knockdown (n = 3 or 5 per group). C) Representative H&E staining results of lung tissues (magnification, 4×, 40×). D) Ashcroft scores of silica‐induced pulmonary fibrosis mice with or without ferritin knockdown (n = 3 or 5 per group). E) Representative Masson's trichrome staining of lung tissues (magnification, 4×, 40×). F) Hydroxyproline content of lung tissue (n = 4 or 5 per group). G) Expression of ECM proteins (Collagen I, Collagen III, and fibronectin) in lung tissue evaluated by Western blot. H–J) Quantitative analysis of Collagen I, Collagen III, and fibronectin protein levels (n = 3 per group). Data are presented as mean ± SD, * p < 0.05, ** p < 0.01, *** p < 0.001. Statistical analysis was performed using one‐way ANOVA followed by LSD (B, F, H‐J) and Dunnett's T3 test (D).

Article Snippet: Approximately 10 mg of lung tissue was weighed and hydrolyzed in 6 mol/L hydrochloric acid at 95°C for 6 h. Hydroxyproline levels were subsequently quantified using a commercial hydroxyproline assay kit (Elabscience, E‐BC‐K062‐M) following the manufacturer's protocol.

Techniques: Expressing, In Vivo, shRNA, Infection, Knockdown, Staining, Western Blot

Journal: BioMed Research International

Article Title: Zerumbone-Loaded Nanostructured Lipid Carrier Gel Enhances Wound Healing in Diabetic Rats

doi: 10.1155/2022/1129297

Figure Lengend Snippet: Hydroxyproline concentration in skin biopsies of diabetic rats treated with NLCG, SSDC, and ZER-NLCG. The concentrations of hydroxyproline were significantly lower in nontreated diabetic than normal rat skin. Among treatments, ZER-NLCG was the most effective in increasing hydroxyproline levels in the diabetic rat skin. Means significantly different from those of (diabetic (nontreatment) control and NLCG-treated rats) ∗ and SSDC-treated rats ∗ B at p < 0.05.

Article Snippet: Wound tissue samples were homogenized in ice-cold tissue lysis buffer and centrifuged at 10,000 × g for 5 min, and the hydroxyproline contents in the supernatants were quantitatively determined by ELISA (CSB-E08838r Cusabio Biotech Co., Ltd., Wuhan, China).

Techniques: Concentration Assay, Control

Journal: Annals of Surgical Treatment and Research

Article Title: Sustained-released mixture of vascular endothelial growth factor 165 and fibrin glue strengthens healing of ileal anastomoses in a rabbit model with intraperitoneal infection

doi: 10.4174/astr.2017.93.3.159

Figure Lengend Snippet: Hydroxyproline concentrations were assessed in the anastomosis on postoperative days 3, 5, 7, and 14. Concentrations were shown in millimeters mercury column (mean ± standard deviation). * P < 0.05, ** P < 0.01, compared with control group. # P < 0.05, compared with FG group. Δ P < 0.05, compared with VEGF group. VEGF, vascular endothelial growth factor; FG, fibrin glue.

Article Snippet: The concentration of hydroxyproline in the anastomotic tissue (wound plus 1 mm of tissue on each side) was detected using Hydroxyproline assay kit (Uscn Life Science Inc., Wuhan, China) by spectrophotometric determination at 550 nm on days 3, 5, 7, and 14 after administrations.

Techniques: Standard Deviation, Control